《植物生理学报》 2010, 46(11): 1135-1139

大豆离体培养及高频再生基因型的筛选

张艳^1,2,3, 南相日^4,*, 满为群^5,*, 李柱刚⁴

1 东北林业大学博士后流动站, 哈尔滨 150040; 2 黑龙江省农业科学院博士后工作站, 哈尔滨 150086; 3 黑龙江省教育学院理 科教育研培部, 哈尔滨 150080; 黑龙江省农业科学院4 生物技术研究所, 5 大豆研究所, 哈尔滨 150086

通信作者：南相日；E-mail: nanxr@hotmail.com, manweiqun@163.com；Tel: 0451-86676104;0451-86668734

摘　要：

为建立一个高效的大豆再生体系用于大豆的遗传转化, 选用 3 个东北主栽品种 ‘ 黑农 35’、‘ 黑农 41’ 和 ‘ 黑农 58’ 的 子叶节和胚尖作为外植体, 分别建立了3 个品种的子叶节和胚尖再生体系, 并研究了6-BA对大豆再生的影响。结果表明, ‘ 黑农41’ 子叶节最适芽诱导培养基为MSB5+1.0 mg•L6-BA+0.2 mg•L-1IBA, 胚尖最适芽诱导培养基为MSB +0.2 mg•L-1 6- BA+0.2 mg•L-1 IBA。‘ 黑农 41’ 再生体系在出芽率、出芽数和芽伸长数上均远高于 ‘ 黑农 35’ 和 ‘ 黑农 58’, 是一个优秀的大豆转基因受体材料。

关键词：大豆; 子叶节; 胚尖; 再生体系

收稿：2010-06-24   修定：2010-09-03

资助：国家转基因重大专项(2008ZX08004-005-02)。

Culture in vitro and High-Frequency Regeneration Genotype Selection of Soybean

ZHANG Yan^1,2,3, NAN Xiang-Ri^4,*, MAN Wei-Qun^5,*, LI Zhu-Gang⁴

¹Station of Postdoctor, Northeast Forestry University, Harbin 150040, China; ²Station of Postdoctor, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;³Department of Science Education Research and Training, Heilongjiang College of Education, Harbin 150080, China; ⁴Biological Technology Research Institute, ⁵Soybean Research Institute, Heilongjiang cademy of Agricultural Sciences, Harbin 150086, China

Corresponding author: NAN Xiang-Ri; E-mail: nanxr@hotmail.com, manweiqun@163.com; Tel: 0451-86676104;0451-86668734

Abstract:

To establish a high frequency regeneration system for the gene transformation of soybean [Glycine max (L.) Merr.], cotyledonary nodes and embryonic tips from three varieties ‘Heinong 35’, ‘Heinong 41’ and ‘Heinong 58’ were used as the explants. Regeneration systems of cotyledon nodes and embryonic tips from three varieties were established in this research. Effect of 6-benzylaminopurine (6-BA) on soybean regeneration was studied. The results indicated that the optimal shooting induction medium of cotyledonary node from ‘Heinong 41’ was MSB₅ added 1.0 mg·L^-1 6-BA and 0.2 mg·L^-1 indolebutyric acid (IBA) and the optimal shooting induction medium of embryonic tip from ‘Heinong 41’ was MSB₅ added 0.2 mg·L^-1 6-BA and 0.2 mg·L^-1 IBA. In comparison with ‘Heinong 35’ and ‘Heinong 58’ regeneration systems, ‘Heinong 41’ regeneration system was superior in shoot regeneration frequency, shoot regeneration number and shoot elongation number. ‘Heinong 41’ was an excellent receptor material in soybeans genetical transformation.

Key words: soybean; cotyledonary node; embryonic tip; regeneration system